By Walker J.M. (ed.), Pollard J.W. (ed.)
CIP cites the sequence because the major access. Covers equipment for growing mammalian fibroblastic phone cultures and keeping tradition stipulations for epithelial, neuronal, and hematopoietic cells between others. recognition is given to the variety of tradition media and extracellular matrices had to retain the differential services of the classy cells. additionally describes tradition recommendations for either residing and stuck cells.
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Extra resources for Animal Cell Culture
4. 8 cell culture supernatant. 43 44 Lymphoblastoid Cell Lines 5. 5% PHA Wellcome. 6. Ficoll-hypaque or lymphroprep. 1. Production 3. Methods of Epstein-Barr Virus Stock (3) 1. 8 cell line (Note 1) is cultured routinely in 5% FBS RPM1 1640. Routine passage consists of diluting the cells between 1 in 2 and 1 in 5 at weekly intervals. A culture in the logarithmic growth phase would usually yield lo6 cells/ml at 37°C. 2. 2 x 106/mL in 2% FBS RPM1 1640. 3. Incubate at 33°C for 2 w without any medium additions or changes.
Mycoplasma infection of cell cultures was first observed by Robinson et al. in 1956 (I). Since then, the incidence of mycoplasma-infected cell cultures has been found to vary from laboratory to laboratory. Currently, 12% of cell lines received by the ECACC are mycoplasma infected, which may be an uncharacteristically low figure since many lines prior to deposition are mycoplasma screened.
Production 3. Methods of Epstein-Barr Virus Stock (3) 1. 8 cell line (Note 1) is cultured routinely in 5% FBS RPM1 1640. Routine passage consists of diluting the cells between 1 in 2 and 1 in 5 at weekly intervals. A culture in the logarithmic growth phase would usually yield lo6 cells/ml at 37°C. 2. 2 x 106/mL in 2% FBS RPM1 1640. 3. Incubate at 33°C for 2 w without any medium additions or changes. 4. Allow the cells to settle out at 4OC. 5. Centrifuge the supernatant at low speed (1508 for 5 min) in order to clarify.